Leadrship Assignment Example | Topics and Well Written Essays - 250 words

Leadrship - Assignment Example tter way to share their ideas and information, they would have to take steps to enhance their communication with their workers, listen to their problems, talk to them and work out their issues. Workers will automatically start following them and the overall organizational culture will improve. This is the transformational leadership actually in which the leaders produce such an effect on their subordinates that they inflict a â€Å"transforming effect† on them thus producing â€Å"sweeping changes in organizations and societies† (Priyabhasini & Krishnan, 2005, p.1). The main reasons I support this concept are that when managers exhibit good listening and communication skills and deal with their workers with respect and dignity, the latter themselves try to step into the former’s shoes by hard work and effort. Also, the overall workplace environment becomes healthy which creates better chances for the sustainability of the company. Thus, managers must know that si nce they are the leaders, they will have to act like parents whom children follow in every action and

International student migration to the UK Essay Example for Free

International student migration to the UK Essay Since the policy of UKs government on immigration dramatically changed in 1997, a positive immigration policy was carried out by the UK government, especially towards temporary skilled immigration (Stam.A, 2006). Thus students migration increasingly became representative of this kind of migration. This students migration trend brings various effects to the UK student immigration pattern as well as the entire UK. Therefore, this essay aims to demonstrate the effect of students migration in China and European country, particularly, analyze the major cause of students migration , then compare the different causes in these two typical examples and evaluate the differences in my perspective. The similar significant effect of students migration in China and European country to the UK is the increasing number of students. According to the report from European Union (EU) after 2004, the A8 countries (from Eastern Europe who joined the EU in the largest single expansion since its creation in 1957) had a great number of increasing students inflow of A8 students to the UK annually (see figure 1). In the academic year of 2008/09 there were approximately 17,000 Higher education students who were domiciled in A8 countries. From this data, it is clear draws the conclusion that the annual students migration at A8 countries were increased. Over the long term, the effects of this immigration pause on the size of European community of education more small. In addition, Non-European country still send large students to the UK. Based on the statistics from UK Higher Education Statistics Agency, during the period of 1995 and 2003, overseas students increase in number from 206,000 to just over 300,000. It claims that overseas students represented over 13% of the entire UK Higher Education student population. (Higher Education Statistics Agency, 2005). In part of Non-European countries student migration, the largest contribution comes from Asia (see figure 2). Figure 1 A8 student numbers in Higher Education institutions in England and Wales, 2001/02 2008/09 (Source: Higher Education Statistics Agency, 2010) Figure 2 Nationality of foreign students entering the UK, 2004-2010 ( Source :Home Office, Control of Immigration :Statistics UK, 2010) However, in terms of the situation in China, the major effect of students migration is not only the increased number of students, but also the financial contribution to the UK government and some negative social effect. According to the survey during 2003-2004, the 43,000 international students from China contributed at least 300 million pounds and increased more 30% than the previous year. Furthermore, this trend still gives rise to the social issues. For instance, some wealthier students might has showed off their high level of consumption which may attract the increased robbery and bring the threat to their safety and even undoubtedly arise the crime rate in local community (Wei .S, 2005). From this case of Chinese students migration, it claims that the effects of student migration have both positive and negative sides. In spite of the effects that European country and China caused, the major reason which causes these effects should be pay more attention to analyze. Just as Mr. Willetts spoken at conference of University UK, Without international students, we could not be only poorer economically-we could also be more boring, more insular, and more ignorant of wider world.(BBC News, 2012). On the one hand, economy drive more Chinese students migration to the UK. After the opening-up Chinese economy reforms since 1970s, many Chinese migrants are chasing the better economic development and have significant migration inflows from China. So students migration becomes the main trend to meet requirements as a consequence of rapid development of economy requires more educated talent in work force. Thus,a great number of parents pull and push their children to study in the UK in order to acquire level of English, study at higher academic reputational university. In contrast, the cause in European country is different. The citizens from A8 country in European started to be implement the fewer restriction on their right entering the UK (Gillingham.E, 2010) and it has reflected on the area of students migration. Specifically, as a result of the more simple process of entering the UK, students are highly attractive source of skilled migration without an excess of limitations. Other causes of difference between China and European country involve issue of visa policy. Overview the Non-European country, the major cause of increase students migration is that student visas are not permanent, and they do not need provide a direct legal route to settlement. On the basic data of entering in the UK, 79% not stayed as British residents and another 6% remained as students who not tend to lead settlement (Achato et al.2010). There extension of visa statement can state that application of Tire 4 Student visas are rising up during the period of year from 2001 to 2010 (see figure 3). China is the largest group of students migration to the UK in Non-European country, so there is no doubt that the visa extension in Non-European country also reflects the same situation in China. Moreover, implementing entry procedures in flexible, provide available service lets students gain work experiences in the UK. This also a effective strategy to keep international intellectual stay in the UK (Wei Shen, 2005). In comparison, students who from European country have fewer limitation about visa. For example, the UK council for International Student Affairs clams that if a student from European Economic Area (EEA), the student could be entitled to the UK freely for up to three months without any demonstration about the right of free movement and do not have to register or apply for any documents in order to stay in the UK.(UKCISA) figure 3 Extension of visas, non-EEA students,2001-10 (Source :Home Office, Control of Immigration: Statistics UK, 2009) To summarize, according to the analysis of these two examples, it is not difficult to state all these students migration have similar effects but different causes of migration pattern. Although excessive student migration can cause some social issues, the similar effect of the increasing number of students immigration to the UK still exist in China and European country as the major impact of students migration. However, the different causes of this migration trend contribute as much as their similar effect. In China, students migration to other nation is to fulfill the developing countrys demand of skilled people to facilitate economy. I believe this cause could attract more students study abroad and so that streamling service of visas application to provide more convenience to students migration. While in European country, there are fewer restriction of visa policy and right entering to the UK than China. This can make the policy about students migration in European country change more to promote the development of it. Therefore, I argue that different causes about students migration in China and European country still cause similar result, and it will be profound to migration pattern in the future. List of References Achato, et al. (2010) The Migration Journey. Home Office Research Report 43.London: Home Office BBC News.(2012,September 13).Overseas students: Change to UK migration figures planned. Available at: http://www.bbc.co.uk/news/education-19579923 Blinder,S.(2011,December). Non-European Student Migration to the UK, The migration observatory, 1st Revision (Next update:12/12/2012). Available at: http://migrationobservatory.ox.ac.uk/briefings/non-european-student-migration-uk Findlay,A.M.and Stam,A.(2006, March 16).International student migration to the UK:Training for the global economy or simply another form of Global talent recruitment? Institute for the Study of International Migration. Available at: http://www12.georgetown.edu/sfs/isim/Event%20Documents/Sloan%20Global%20Competition%20Meeting/Findlay-UK.pdf Gillingham,E. (2010, November). Understanding A8 migration to the UK since Accession, Office for National Statistics. Available at: www.ons.gov.uk/ons/rel/migration1/migration-statistics-quarterly-report/novem ber-2010/understanding-a8-migration-to-the-uk-since-accession.pdf Higher Education Statistics Agency (2005). Students in Higher Education Institutions 2003/4.(also 1994/5-present) The Higher Education Academy (2007). The internalization of UK Higher Education: a review of selected material. Available at: http://www.heacademy.ac.uk/resources/detail/the_internationalisation_of_uk_he UK council for International Student Affairs(no date). EEA and Swiss students. Available at: http://www.ukcisa.org.uk/student/eea.php#register Wei Shen (2005). A study on Chinese student migration, Asia Europe Journal, 3(3). Available at: http://www.springerlink.com/content/q8988416j37t6144/

Business plan for a fruit processing company

Business plan for a fruit processing company Our Business Plan will be about fruit processing. This will reduce fruits post harvesting loss through processing fruits in syrups and concentrates. This will help middle and low income eaners as we find there is high need of fresh fruits juice as many of fresh fruits juice are imported and sold in high price which normal Tanzania citizen cant afford. Our company will be situated in Korogwe Tanga where there are many firms. Company has three partners, Mildred, Sophia and Saja. ABRIVIATION ASDP Agricultural Sector Development Programme ASDS Agricultural Sector Development Strategy BIT Board of Internal Trade DACHE Dar Es Salaam College of Home Economics Ltd GDP Gross Domestic Product HKMU Hubert Kairuki Memorial University MAFC Ministry of agriculture Food Security and Cooperatives MITM Ministry of Industries Trade and Marketing NARS National Agricultural Research Institute PASS Private Agricultural Sector Support SIDO Small Scale Industries Development Organization SIDP Sustainable Industry Development Programme SUA Sokoine University of Tanzania TDV Tanzania Development Vision TFDA Tanzania Food Drugs and Cosmetics Agency UDSM University of Dar es Salaam BUSINESS PLAN 1.0 Executive summary The current agriculture potentiality contributes about 26.5% of GDP and it provides employment about 70% of total labour force. Tanzania grows a range of fruit including topical and temperate. The annual production of fruits is 678,515MT (this is 5% of potential area exploited) [tanserve n.d] Agro processing in the industrial sector is indeed one of the underdeveloped sub sectors and according to a report by Commonwealth Secretariat (1997) it was seen to consume about 1% of the horticultural raw materials produced in Tanzania as compared to 80% in Malaysia which also is in the category of developing country [practicalaction n.d] Agro processing and especially small -scale processing is becoming very important in the country as means to reduce crop losses and value addition at production and collection sites and a way of income generating activity.[practicalaction n.d] However, the sector experience losses during and after harvest. Post harvest losses of fruits and vegetables are estimated at 50 to 80%. These losses are more meaningful economically compared to the cost of compensating for losses through increased crop production. Losses are caused by among another factors, mechanical, physical and biochemical aspects. SOMIL intends with this financial and economical projection the idea will bring new concept in a market and good strategies which will enable the company to acquire a good market position initially the company may have up and downs of new start but with the partner potentiality gradual the sales may increase once SOMIL gets good eyes to watch over it operations. A daily production of between 2000 and 3000 kilograms of fruit product is estimated to be obtained from an input of between 2000 and 3000 kilograms of fruit pulp/product is estimated to be obtained from an input of 5000 kilograms. Production time per annum is estimated to be 240 days. And the project intends to employ 22 people and it is an investment of Tshs. 300,000,000.00 which will realize Tshs 144,000,000.00 in year five times. Total production costs are estimated at Tshs. 290,000,000.00 in year one gradually rising in years two and three then falls in year five onwards. Project cash flow is favourable, it will start making profits from the first year of the project and all loans be repaid by third year of operation. This will be showing clearly in financial. 2.0 THE BUSINESS 2.1 The opportunity According to government record and statistics show the annual production of fruits to be 678,515MT, but this is only 5% of the potential area exploited and this can be caused by lack of where to send the increased production. 2.2 Need to be filled This project so as to say will focuses on increasing fruits usability which experience enormous looses especially during the season and create new opportunities for industrial development in rural areas through processing of fruits for fresh juice. More so, the initiator of this idea is a professional expert in the field of post harvest processing, having a long term experience in offering knowledge to small scale food processors and food enterprises and managed to make them grow to a medium scale capacity. 2.2.1 How the proposed business will fills the need With the capacity of operation which business plan to operate in, the company will process fruits for juice which will be consumed by low and medium income earners who are not able to buy exported fresh fruit juice. 3.0 Company and Company history Initially idea was brought by one of the partner who is professional in a filed and she has long term experience in food processing later on the idea was joined by two other partners who contribute in capital invested. 3.1 Company mission statement To provide 100% satisfaction by offering fruit in syrup and fruit concentrates of the highest quality, impeccable services, guaranteeing accurate supply, fast delivery and developing strong relationships with farmers and medium to small scale fruits processing business and entrepreneurs. 3.2 Our vision SOMIL forecast to be competitive in service while taking on prior responsibilities that are pertinent to our clients needs through producing strict quality and safe products, for health and economical satisfaction. 3.3 Company objective The products to be made include quality and safe fruits of the season concentrates and fruits in syrup. Neutrality will govern the products made since raw materials will basically comprise of fresh, mature fruits of the season without non-fruit fortification. SOMIL products are intermediate since they form raw material to juice making, wine making and fruit salads to other small to medium scale industries. Availability regardless seasonality forms another niche value to SOMIL products. The main objective is to ensure sustainable economic, food and nutrition security by seasonal reduction of fruits post harvest loses by 10% through processing. 3.4 Specific objectives To ensure the establishment of fruit concentrate and fruits in syrup industry is in place by end of year one of the project inception To establish appropriate links with stakeholders who will daily deliver to the industry quality seasonal fruits for processing To continuously encourage and support value addition to seasonal fruits so that the farmers are economically empowered Together with farmers and other stakeholders of the project area, to reduce the post harvest losses by 50% through fruits processing by year 2013 To continuously support technology developments in the post-harvest system according to zonal conditions, climatically, and socio-culturally and according to the needs of the clients for effective and sustainable availability of the industrys raw material. 4.0 COMPETITIVE ADVANTAGE Fruit concentrates and fruits in syrup are made from fresh fruits of the highest quality, maturity, and health from both temperate and tropical zones. The fruits have to be selected and graded through a rigorous quality control system so that all of our products have the best flavors and nutrient content that meet the national and international quality and safety parameters and standards. The products made by SOMIL will be raw material to other secondary fruit products that include among others; juices, wines, fruit salads and fruit based products. Bulk packaging will govern the products and minimum carriage weight will be 20 kilogram and maximum 200 kilogram drums. Literature review and a study visit to few industries in Dar es salaam indicates about 16medium scale and 300 small scale prospective competitors, however, paste, fruit concentrates and fruits in syrup are not their core activities to any of these companies. They specialize in finished products of this semi finished products. Similarly, the demand for SOMIL products especially during off seasons in which consumers shift to imported pastes and fruit concentrates is prospectively high. Furthermore, customers of secondary products (finished products) have become health conscious, which add to the unique selling point of the company products. The company is reputed of having competent employees both professionally and aggressiveness to deliver as per vision of the company. 4.1 Description of the business model The company will deliver its products in the first come first served basis. Since nature of business entail quality and safe semi finished products than enterprises of finished products will certainly find them highly convenient both as a time saver as well as all-seasons products. Despite the presence of few competitors the demand is rarely met, also they utilize mostly the imported concentrates that are generally fortified with non fruit material thus reducing the products nature and quality. Consumers of the finished products have become health conscious which adds to the unique selling point of SOMIL products as they are naturally made from whole fruits and vegetables with nothing added hence making the product whole, fresh and nutritional. The company is reputed of having competent employees both professionally and aggressiveness to deliver as per vision of the company. 4.2 How the business will create a sustainable competitive advantage Production cost govern to a large extent pricing strategy but also comparisons have to be made with competitors prices so a not to be very different for a product of similar or near similar. Other quality parameters such as health impact, intellectual property etc will also be considered in the setting. According to studies on food habits conducted by use of a case study of Dar es Salaam, consumers reported to be willing to pay a little more for a healthy product especially when they are ensured of its quality and safety (Mjawa, 2003) For this reason, if a small price difference is set (ceteris peribus), it will make the business sell few volumes of quality products yet with profits till it gains market reputation. 5.0 CURRENT STATUS AND REQUIREMENTS The location of the production area will be Korogwe town and marketing office will be Korogwe District Tanga region. The choice of a production location is strategically due to the place being the major centre of fruits growing with a horticultural hinterland. Besides Korogwe being centrally located it allows easy transportation of inputs and raw materials to the industry as well as products to the selling centre and consumers due to its proximity to most areas. The location will also tap the availability of labor that has been left redundant by many crop estates and industries that were closed and some are now affected by the world economic recession. Ample availability of steady services like ICT, electricity, and water are taken into consideration when selecting the location. Taking into consideration that this is a start up project, the following will form a production and operation processes. Table 2: Production process Step Process Description 1 Site acquisition, registration of business and other start up logistics Site us owned by one of the partner which will be transfer in the business under the law and legal contract. 2 Purchase of equipment Machineries equipment with the capacity of producing 500-800 kilograms per hour will be procured. Office equipment, a vehicle and furniture will also be procured. 3 Employees recruitment Director production and technical, manager administration and finance, manager production and technical will be the first people to be employee operators staff and hiring the security. 4 Purchases of production materials Identification of locations, suppliers of raw materials and other inputs; training on the quality to supply and later purchases to be done 5 Production process Receiving, grading, washing, desizing/pulping, packing packaging and storing 6 Marketing Market research, Transportation, advertisement, selling. 5.1 Description of where the business stands today SOMIL has 120 millions which contributed by three partners in equal shares also the company has plot in Korogwe which has own by one of the partners and transfer to a business through legal entities. 5.2 Description of what the business needs to move forward In order the company to operate successfully the business needs 300 millions. Where by 120millions has already contributed by three partners and remained 180 millions the company look for a loan which will be paid within five years. 6.0 MANAGEMENT The business is directed by three directors, who are partner and shareholder of the business. First partner is MILDRED MLAKI, who has two degree, one from Cape Town University of food and nutrition and the other is for commerce from university of Dar es salaam, Mildred will be overall in-charge of all processing operations. Second partner, SOPHIA MUSSA holder masters in human resource from makerere university of Kampala Uganda, she is working with reputable company in the country and she will be overall in-charge with human resource and public relation of the company. Third partner, SAJA JOHN is a holder of masters in strategies business from Greenwich University; she will be overall in-charge of market operation of the company. The company will employ other work 2 in finance and procurement 2 foreman, and production and technical will be hired and security on term for six month period to help initial stage of layout, installation and commissioning of the machines and equipment. 6.1 ORGANISATION STRUCTURE 6.2 Ownership The business is currently owner by 3 directors as mentioned above and the will contribute 60% of the initial investment and the rest may be sold to any interested investors or development partner. 6.3 Board of Directors/Board of Advisor A Board of Advisor will be selected to advice on matters regarding smooth running of the business based on qualification specific to the organization functions. Advisors will strictly come from research institutes like UDSM Process Engineering Department, SUA, NARS, and TBS etc since the business needs technical expertise and solutions from them for the benefit of making it outstanding in terms of quality and up to date with technological features. A political figure will be included for the health of the business; the main proposal is the Member of Parliament for Korogwe Constituency. 6.4 Key Professional Service Providers The services of a change agent like SIDO experts are vital for advising on entrepreneurship skills including business management and capital acquisition. The service of reputed audit personnel for legal advisor is required by the business, but company will higher advocate professional whenever needed. 7.0 INDUSTRY ANALYSIS The current agriculture contributes about 26.5% of GDP and it provides employment to about 70% of total labour force out of which 56% of them are women. (Human Development Index 2007). This is because of its subsistence nature of Tanzania Agriculture but its potentiality for agriculture growth is immerse taking into the focus of the government strategies. TDV (Tanzania development vision) and ASDS that envisage to transform the existing subsistence-dominated agriculture progressively into commercially profitable and viable production systems so as to make it modernized, commercial, highly productive and profitable by utilizing new technologies and available technologies and manpower in an overall sustainable manner. (www.tanzania.go.tz) Agro processing in the industrial sector is indeed one of the underdeveloped sub sectors and according to a report by Commonwealth Secretariat (1997) it was seen to consume about 1% of the horticultural raw materials produced in Tanzania as compare to (80%) in Malaysia which also is in the category of developing country. Also according to PASS (2002), 65% of the processed products are consumed within the country. This indicates the availability of investment opportunities in the fruits and vegetable processing (www.tanzania.go.tz) Most of the processing plants operate under capacity due to old technology used, irregular supply of raw materials and low technical skills. However, there is some investment in processing industry taking place and efforts are being made to promote more investment in this area. Small-scale processing in becoming very important in the country as means to reduce crop losses and value addition at production and collection sites, and a way of income generating activity. To date over 5,700 processors have been trained and at least 3000 jobs created (SIDO, 2007). The future discourse for industrial development in Tanzania is elaborated in the Sustainable Industrial Development Policy SIDP. The main purpose of SIDP (Sustainable Industry Development Programme) is to set out a path for industrializing Tanzania so that by the turn of the first quarter of the 21st Century is becomes a semi industrialized country. Recently, individuals and or groups of women entrepreneurs and youth have engaged themselves into processing of fruits and vegetables for niche markets. These groups have received basic training in food processing technology through research institutions such as TIRDO (Tanzania Industrial Research Development Organization, TFNC (Tanzania Food Nutrition Centre) SUA (Sokoine University of Agriculture), Agriculture Research Institute Uyole, Mbeya Horticulture Research Training Institute Tengeru, Small scale Industry Development Organization (SIDO) and non-government organizations supporting community, nutrition and health improvement programmes. Such groups are mainly engaged in drying of fruits like ripe bananas, pineapples and mangoes and traditional vegetables such as amaranths, cassava leaves, okra and cowpea leaves. Others are engaged in making products like juices, jams, marmalades, pickles, tomato sauce and wines. (www.tanzania.go.tz) SWOT ANALYSIS TO OUR STARTUP BUSINESS STREGHTHS Highly experienced technologically Willingness to deliver as per vision Strong team of management WEAKNESSES Limited capital to invest in large scale venture A less reputed venture not yet gained a name OPPORTUNITIES Niche markets that take advantage of seasonal shortages Consciousness of fresh and nutritional healthy products by consumers Availability of ample and variety of fruits in most time of the year Location is along the fruits and vegetable growing zone Location centrally located, feasible transport wise to both farmers and consumers Availability of many funding arrangements like loans and grants for investors and entrepreneurs. Recent economic recession will reduce competing imports to a large extent THREATS Importation of less quality but cheap concentrates Emerging large scale investors with similar project Weather calamities such as drought that may hamper production Inconsistent supply of raw materials due to seasonality Substitutes such as Soda and Mineral water especial Uhai, Kilimanjaro, Dasani, masafi and so on 7.1 Competitive position within target market 7.2 Competitors analysis Few medium to large scale industrial processing plants in Tanzania that are currently operational, are located in strategic areas of high production and/or consumption like Bakhresa Food products, Noble Foods beverages Ltd, A-One products and Bottlers Ltd and Redgold in Dar es Salaam region, Darsh Industries Ltd in Arusha region, Dabaga Fruit and Vegetable Caning Factory in Iringa region. These factories produce a range of products that include fruit juices of various kinds, tomato sauces, pickles, wine, ketchup, etc. Other factories are emerging in other potential areas like in Morogoro as more investors venture into this sub-sector. (www.tanzania.go.tz) Table 1. The following table indicates few of the prospective competitors. Name of competitors Product made Strengths Weaknesses Remarks that makes SMS to capture a market share Noble foods-fruits juices Mango juice Fresh mangoes during the season. Have good packages No production during off season, manufactures only one product -mango juice Potential customer during off season may purchase other fruit pulps for SMS Bakhresa Food Industries -|Azam fruits juices Juices: mango, guava, apple, pineapple oranges May not lack raw material due to utilizing imported concentrates, few fresh fruits. Utilizes all the raw material in his own industry Although a big competitor but not threatening because he cannot sell to others hence consumers of SMS are maintained Red Gold Tomato products Sauces and Chill Uses fresh tomatoes during the season, import during off season Do not stock enough for off season production Potential competitor and customer Small scale wineries, juice processors Tomato products, wines, juices and fruit blend recipes Uses fresh fruits of the season Normally do not stock enough for the year. Have little funds and small premises for keeping large amounts of raw materials May be competitors during the season but big customers during off season Other medium and small scale industries Tomato products, wines, juices and fruit blend recipes Uses fresh fruits and vegetables as raw materials to their secondary products Cannot stock due to capital, convenience, expertise and space Potential customer Talking about competitors also there is a threat of substitute such and Soda and mineral water. 7.3 Market size and Trends According to the Confederation of Tanzania Industries directory (2007), the total market is about 316 consumers for these products. The business estimates to capture 10% in the first year 20% in the second year and growing to 30% in year five onwards. This will be due to gaining faith regarding quality as compared to imported products. The business administration will take care of customer training building regarding confidence building. Regarding a study by Tanzania Food Drugs and cosmetic Agency (TFDA) on Inventory of Food industries (2006), medium and small scale industries that will utilize SOMIL products are concentrated in Arusha, Moshi, Dar es Salaam, and Tanga regions and most of these utilize the product as raw material for making secondary products such as sauces, wines and juices. Government efforts to strengthen the increased production of non traditional crops such as fruits and vegetables because they are of high value to both farmers and the country impact positively to the availability of raw materials for SMS products and hence ensure readily availability of raw material for SMS products and hence ensure readily availability of products to clients. The world economic trends may have a positive impact on customers products due to reduction of imported concentrates. 8.0 MARKETING PLAN 8.1 Market Summary SOMIL fruits product will possesses good information about the market and known a great deal about the common attributes of the most prized customer. This information will be leveraged to better understand who is served, what their specific needs are and how SOMIL can better communicate with them. [Kotler P Keller KL1956, P.61-67] 8.2 Marketing strategy The initial focus will be locally where small scale and medium fruits processing industries are located. Due to capacity of the industry at the moment, there are no plans to go beyond Tanzania and Zanzibar. The strategy will initially be identification using data from Board of Internal Trade (BIT), the Ministry of Health and Social Welfare-TFDA and Ministry of Industries Trade and Marketing (MITM) as well as Tanzania Bureau of Standards (TBS) and other trade newsletters. The selected few will be visited for introduction and sample sending. Then a business may further advertise in public media newspapers, radio leaflets distributed to road users for know. In future we will advertise at Televisions also. 8.3 Concept testing Concept test system can improve the company chances of success in developing and introducing new products. Our organization will go door to door for the concept test in order to get the view of the public concerning with our types of product; this will be done in public area like schools, Hospital and churches. Design employs sequential public study in which up to 50 groups of 10 to 20 participants each test one product. These studies typically consist of three surveys of up to 30 questions each. The surveys are conducted among members of our Members. 8.4 Usability testing Usability testing is a technique used to evaluate a product by testing it on users. This can be seen as an irreplaceable usability practice, since it gives direct input on how real users use the system. This is in contrast with usability inspection methods where experts use different methods to evaluate a user interface without involving users. Usability testing focuses on measuring a human-made products capacity to meet its intended purpose. Examples of products that commonly benefit from usability testing are foods, consumer products, How ever in organization will used this test to improve our product in this areas efficiency, accuracy, recall, and emotional response. The results of the first test can be treated as a baseline or control measurement; all subsequent tests can then be compared to the baseline to indicate improvement. This test will assist the industries to know where we are going and what people says about our products, This test will be assist us to maintain our product and make it durable in all time for production, our organization will insure this test done before the product enter in the market. 8.5 Channel of distribution 8.5.1 Selecting a channel of distribution SOMIL when selecting a channel of distribution as Fruit product Industry will consider the follow: The consumer characteristics need and segment The company goals, resources expertise and experience The product value, complexity, perish ability and bulk The competition characteristics and tactics Distribution channels alternatives, characteristics and availability Legalities current law and pending laws. [Kotler PKeller KL1956, P.36-72] 8.5.2 Distribution standard SOMIL will have clear and measurable goals regarding service levels in physical distribution. In so doing there must be a clear distribution strategy for products to reach the target consumers, in this respect the clear distribution channel must be in place. 8.5.3 Customer Services SOMIL will understand the decisions involve delivery frequency, speed and consistency transportation and shipping to policies, whether to accept small customer orders; warehousing coordinating assortments; and so on. Poor performance in these areas may result in lost customers. [Kotler PKeller KL1956, P.36-72] 8.6 Production 8.6.1 Social responsibility. SOMIL we will consider the impact of companys action and operating in a way that balances short term profit needs with societies long-term need this ensuring the company survival in a health environment. 8.6.2 Natural resources SOMIL will make sure that the depletion of natural resources can be reduced if the consumption of scarce material is lessened and more efficient alternatives are chosen. 8.6.3 Environmental Pollution SOMIL will make sure that dangerous pollutants need to be eliminated from the environment and safe substitutes found. The environment protection is the major federal organization involved. [Ethical Partnership nd] 8.6.4 Child labour SOMIL we will make sure there is no possibility of child labour. Sometimes supervisors use this opportunity to pay this child less while they benefit against them. We will obey the law and perform as per given instructions. 8.6.5 Marketing SOMIL as a company which deal with products fall under the umbrella of the Marketing Mix (product, price, place and promotion) which describes the strategic position of a product in the market place. An enterprise involved in producing fruits products must have clear strategies of producing those products. Also the firm dealing with fruits production should have clear pricing strategies, promotion strategies and placing or distribution strategies. An enterprise we must be able to market what we produce and produce what we can market. Consumerism SOMIL fruits product manufacturer we agree that customer has right to be informed and protected against fraudulent, deceitful, and misleading statements, advertisement, labels Consumer safety SOMIL fruits product manufacturer we will make sure that, consumer are protected against dangerous and unsafe product. Consumer information and education SOMIL fruits product manufacturer we are aware that, consumer have right to be informed includes protection against fraudulent deceitful, or grossly misleading information advertising, labeling, pricing, packaging or other practices. Consumers choice SOMIL fruits product manufacturer we know that consumer has a right to choose means that consumers have available several products and brands for which select. Consumers right to be heard SOMIL fruits product industry, consumers has right to be heard means that consumers should be able to voice their opinions to business, government and other parties. This gives consumers input into the decisions that affect them. Advertisement/Promotion Promotion and advertisement our product should be an almost unconscious part of our everyday sales plan. We dont have to look far for an opportunity to market and merchandise juice. A great way to stand out from the competition is to produce our own juice, thereby offering customer juice beverages that cant be found elsewhere. SOMIL we have to understand our competitors, what they doing and we will take action against these competitors, especially as an entire generation or retail buyers is at stake. To reach the public, advertising is a necessity. We believe that advertising may reach non-juice drinkers and thus bring in additional customer to our business. Advertising deserves greater consideration as a part of the solution to certain

Theories of domestic violence

Theories of domestic violence There are many different theories as to the causes of domestic violence (abuse). These include psychological theories that consider personality traits and mental characteristics of the offender, as well as social theories which consider external factors in the offenders environment, such as stress, social learning and drug and alcohol abuse. Psychological theories focus on personality traits and mental characteristics of the offender. According to this theoretical approach, characteristics associated with individuals who abuse their partners include low self-esteem, isolation from social support, a manipulative nature, and a desire for power and control (Suman Kakar 1998). These individuals are likely to be unwilling to take responsibility for their own actions, have extreme feelings of jealousy and possessiveness, be overly dependent on the victim, and/or have certain mental or psychological disorders. An important aspect in the psychological theory is power and control. In some relationships, violence arises out of a perceived need for power and control. This is where the abuser may use violence as a strategy to gain or maintain power and control over the victim. Abusers may feel the need to control their partner because of difficulties in regulating anger and other strong emotions, or when they feel inferior to the other partner in education and socioeconomic background. For instance, in our society today, women have moved away from being just a housewife and taken up the role as a career woman. No longer are women staying home and tending to the house while men go out and work. In fact, a lot of women have taken over jobs that were previously held my men (women politicians). This has brought about a power struggle in the family which often leads to domestic disputes and abuse: Some men with very traditional beliefs still think they have the right to control women, and that women are not equal to men, while women on the other hand, are vying for power and control. Stress may be increased when a person is living in a family situation, with increased pressures. Social stresses, due to inadequate finances or other such problems in a family may further increase tensions. Violence is not always caused by stress, but may be one way that some (but not all) people respond to stress. Families and couples in poverty may be more likely to experience domestic violence, due to increased stress and conflicts about finances and other aspects. Some speculate that poverty may hinder a mans ability to live up to his idea of successful manhood, thus he fears losing honor and respect. As a result of him not being able to economically support his wife, and maintain control, he may turn to violence as ways to express masculinity. Social learning theory suggests that people learn from observing and modeling after others behaviour. With positive reinforcement, the behavior continues. If one observes violent behavior, one is more likely to imitate it. If there are no negative consequences (e.g. victim accepts the violence, with submission), then the behaviour will likely continue. Oftentimes, violence is transmitted from generation to generation in a cyclical manner. According to Faith St Catherine of the Womens Resource and Outreach centre in Jamaica, there is a culture of abuse, especially among the inner city poor Studies have found that nearly one half of abusive men grew up in homes where their father or step father was an abuser. An environment where violence is either taught, by example, or accepted as normal will imprint upon a childs psyche. For instance, a young boy may see his father come home from work drunk and angry, screaming at his mother. He watches his mother attempt to please and placate his f athers drunken behaviour. The young boy is being taught that violence gets results. He is developing his own ideas about what makes a man. http://books.google.com/books?hl=enlr=id=BPT0HelrVcMCoi=fndpg=PA201dq=Social+theories+of+Domestic+Violenceots=9iVvl8_Tprsig=C9P8UBogyad2RePEnpeTk5JYs7Y#v=onepageq=f=false Drug and/or alcohol abuse may be a precursor to domestic violence. Substance abuse leads to out-of-control behaviour. A drunk or high person will be less likely to control his or her violent impulses. However some have argued that abusers use drug and alcohol as an excuse for their action. Yet, alcohol is an important risk factor for partner abuse. According to University of the West Indies professor and gender expert in Trinidad, Rhoda Reddock: in Trinidad, many of the most gruesome murders and sexual violence are linked to mental disease brought about by drug and alcohol addiction, respectively. Since alcohol decreases control and raises the potential for acting on impulse, it is not surprising that some feel it can be a catalyst for abuse. Often a person is able to maintain control of violent emotions when he is sober, but after a few drinks, he becomes abusive. The alcohol has dulled his wits and diminished his ability to control his temper. In the Caribbean or more specifically in Barbados, domestic violence is becoming more and more apparent in the society. Domestic violence is seldom reported in the island, hence why the true incidence of domestic violence is unknown. In November of 2005, according to The World Health Organization (WHO) one woman in every three (3) women are reported to be sexually abused during childhood or adolescence. The study revealed that the most common forms of violence is meted out by loved ones. As mentioned earlier, domestic violence is also known to be closely linked with drug and alcohol abuse. According to Tessa Chaderton-Shaw , manager, of the National Council of Substance Abuse (NCSA), There are many cross-cutting issues with substance abuse and domestic violence She also stated that, It can lead to isolation, shame, guilt, initial denial, loss of support, low self-esteem and a potential for criminal involvement. People then became more aware of Domestic Violence in the country, and th e awareness has constantly been growing. Even the Barbados Police Force has taken domestic violence under more serious consideration and had devised a strategic plan to address domestic violence and reduce its occurrence, according to Sergeant David Wiltshire. Wiltshire said that officers were sent to the United States and England for training to respond to domestic violence issues. References Theories http://social.jrank.org/pages/210/Domestic-Violence-Causes-Domestic-Violence.html http://en.wikipedia.org/wiki/Domestic_violence#Psychological http://wost201h_domviol.tripod.com/groupactionproject/id4.html Suman Kakar Criminal Justice Approaches to Domestic Violence (1998). Rhoda Reddock Faith St Catherine http://www.jamaicaobserver.com/magazines/AllWoman/html/20061203T000000-0500_116180_OBS_CARIBBEAN_FACES_DOMESTIC_VIOLENCE_CHALLENGE_.asp Barbados Domestic Violence http://archive.nationnews.com/archive_results.php?mode=allwordsIncludeStories=1numPer=20start=0keyword=Domestic+ViolencesmartText

Does King Lear Play The Tragic :: essays research papers

Does King Lear Play the Tragic Hero, or the Autocrat? It is quite possible to make an argument in favour of either answer, an argument that would prove to be quite a debate, although one answer would weigh in favour of the other. To prove this, certain elements would have to be analysed thoroughly, all aspects taken into context and sufficient research done into the matter. This is the only method in which a fair debate of the argument can be taken into consideration. We can only find the answer to this question if we know what the two arguments mean; this will provide a solid base onto which the rest of the debate can rest, a foundation of fact. Aristotle, a great founder of the definition of tragedy used the word catharsis to describe the effects of true tragedy on the spectator. Aristotle stated that the purpose of tragedy was to invoke pity and terror, and thereby effect the catharsis of these emotions. Other critics see tragedy as a moral lesson in which fear and pity are excited by the tragic hero’s fate serve to warn the spectator not to similarly tempt providence. This interpretation is generally accepted that through experiencing fear vicariously in a controlled situation, the spectators own anxieties are directed outward, and, through sympathetic identification was the protagonist, his insight and outlook are enlarged. Also, as importantly and significantly, Aristotle introduced the term hamartia, the tragic flaw, or an inherent defect or shortcoming in the hero of a tragedy. Aristotle casually described the tragic hero as a man of noble rank and nature whose misfortune is not brought upon him by villainy or corruption, but by some error of judgement. This imperfection later became known, or interpreted as a moral flaw, although most great tragedies defy such a simple distinction of the term. We could say that in many cases of tragedy the hero is never passive, but struggles to resolve his tragic difficulty with an obsessive dedication, that he is guilty of presuming that he is godlike, attempting to surpass his own human limitations. The need, or lack of order in a society, could be a reason why the tragedy came to be, and is known in Greek terms as hubris. This ethical and religious thought portrays the resulting implications of impious disregard of the limits governing human action in an orderly universe. It is the sin to which the great and the gifted are most susceptible, and in Greek tragedy is usually the hero’s tragic flaw.

Act 3, Scene 1 of Julius Caesar :: essays research papers

Act 3, Scene 1 Rome. Before the Capitol; the Senate sitting above. [ previous scene ][ home page ][ next scene ] A crowd of people; among them ARTEMIDORUS and the Soothsayer. Flourish. Enter CAESAR, BRUTUS, CASSIUS, CASCA, DECIUS BRUTUS, METELLUS CIMBER, TREBONIUS, CINNA, ANTONY, LEPIDUS, POPILIUS, PUBLIUS, and others CAESAR [To the Soothsayer] The ides of March are come. Soothsayer Ay, Caesar; but not gone. ARTEMIDORUS Hail, Caesar! read this schedule. DECIUS BRUTUS Trebonius doth desire you to o'erread, At your best leisure, this his humble suit. ARTEMIDORUS O Caesar, read mine first; for mine's a suit That touches Caesar nearer: read it, great Caesar. CAESAR What touches us ourself shall be last served. ARTEMIDORUS Delay not, Caesar; read it instantly. CAESAR What, is the fellow mad? PUBLIUS Sirrah, give place. CASSIUS What, urge you your petitions in the street? Come to the Capitol. CAESAR goes up to the Senate-House, the rest following POPILIUS I wish your enterprise to-day may thrive. CASSIUS What enterprise, Popilius? POPILIUS Fare you well. Advances to CAESAR BRUTUS What said Popilius Lena? CASSIUS He wish'd to-day our enterprise might thrive. I fear our purpose is discovered. BRUTUS Look, how he makes to Caesar; mark him. CASSIUS Casca, be sudden, for we fear prevention. Brutus, what shall be done? If this be known, Cassius or Caesar never shall turn back, For I will slay myself. BRUTUS Cassius, be constant: Popilius Lena speaks not of our purposes; For, look, he smiles, and Caesar doth not change. CASSIUS Trebonius knows his time; for, look you, Brutus. He draws Mark Antony out of the way. Exeunt ANTONY and TREBONIUS DECIUS BRUTUS Where is Metellus Cimber? Let him go, And presently prefer his suit to Caesar. BRUTUS He is address'd: press near and second him. CINNA Casca, you are the first that rears your hand. CAESAR Are we all ready? What is now amiss That Caesar and his senate must redress? METELLUS CIMBER Most high, most mighty, and most puissant Caesar, Metellus Cimber throws before thy seat An humble heart,-- Kneeling CAESAR I must prevent thee, Cimber. These couchings and these lowly courtesies Might fire the blood of ordinary men, And turn pre-ordinance and first decree Into the law of children. Be not fond, To think that Caesar bears such rebel blood That will be thaw'd from the true quality With that which melteth fools; I mean, sweet words, Low-crooked court'sies and base spaniel-fawning. Thy brother by decree is banished: If thou dost bend and pray and fawn for him, I spurn thee like a cur out of my way. Know, Caesar doth not wrong, nor without cause Will he be satisfied. METELLUS CIMBER Is there no voice more worthy than my own To sound more sweetly in great Caesar's ear

Biology Lab

Lab   #1   ? Introduction   to   the   Microscopy   & Observation   of   Prokaryotic   and   Eukaryotic Cells Introduction Many   of   the   cells   and   organisms   that   you   will   be   studying   are   at   the   lower   limits   of   visibility   of   light   microscopes;   therefore,   it   is   extremely   important   that   you   attain   critical   lighting   and   focussing. It   is   also   important   to   handle   the   microscope   competently   to   avoid   damaging   either   the   microscope   or   the   preparation   you   are   studying. Even   students   who   have   previously   used   microscopes  should  read  the  instructions  carefully. Guide  Biolabo Using a web rowser, go to   the   following   web   site: http://salinella. bio. uottawa. ca/biolabo/   (you   can   try   it   from   home). Under   Microscopy   you   will   find   links   to   pages   that   describe   both   type   of   microscopes   you   will   use   this   semester,   as   well   as   how   to   set   up   and   use   them. It   is   strongly   recommended   that   you   visit   these   pages   prior   to   attending  your  first  lab. Image  J  /  Qcapture Although   you   can   make   all   your   observations   by   watching   directly   through   the   oculars,   it   also   can   be   done   on   the   computer   screen   using   the   digital   camera   attached   to   each   microscope.For   that,   you   will   use   the   Image   J   program   together   with   a   capture   plugin called   Qcapture. Visit   the   lab   website   to   learn   how   to   use   Image   J   (link   on   the   homepage). All   observations   can   be   made   on   your  computer   screen   or   in   the   oculars. Each   method   has   its   advantages   and   drawbacks;   you   will   have   to   choose   which   one  it  more  appropriate  (or  the  one  you  prefer): Oculars Screen ? Greater  resolution ? Wider  field  of  view ? Can  share  observation  with  others ? More  comfortable  for  users ? Take  pictures  while  observing Lab1  ? Microscopy The  Compound  Microscope On   the   Guide   Biolabo   page   click   on   the   CX41   Compound   Microscope   link   then   on   Parts   and   Function. This   will   bring   up   a   labelled   line   diagram   of   your   microscope. Familiarize   yourself   with   the   various   components   shown   in   this   figure. Then,   click   on   Setup   and   Bright   field   alignment   in   order   to   know  how  to  use  and  handle  the  microscope. Now,   locate   your   compound   microscope   in   the   cupboard   below   the   sink   of   your   workstation. Place   it   on   the   counter   between   the omputer   and   the   end  of  the  counter. Be  sure  that  whenever  you  transport  the  microscope,  it   is   always   kept   upright;   the   ocular   lens   will   fall   out   if   the   scope   is   tilted   or   swung. Even   though   you   don’t   need   the   dissecting   microscope   right   now,   take   it   out  of  the  cupboard  and  install  it  beside  the  compound  microscope. Connect   one   firewire   cable   to   each   of   the   cameras   installed   on   top   of   the   microscopes. This  way,  everything  is  setup  for  further  observations  both  on   your  computer  screen  and  through  the  oculars. Parts  of  the  compound  microscopeThe   microscope   consists   of   a   system   of   lenses,   a   light   source,   and   a   geared   mechanism   for   adjusting   the   distance   between   the   lens   system   and   object   being   observed. There   are   a   number   of   important   components   and   it   is   essential   that   you   be   able   to   identify   them   and   understand   their   function   before  you  can  proceed. By  going  through  the  different  modules  in  Biolabo   and   using   the   microscope   you   will   develop   a   competency   for   bright   field   microscopy. Identify   the   following   components   using   Biolabo   (Parts   andà ‚   functions   figure)  and  your  microscope:REVOLVING  NOSEPIECE:  Supports  the  various  objectives  ? You  will  only  use   the  4x,  10x  and  40x  objectives  in  the  BIO1140  labs  (not  the  100x). STAGE:   Supports   the   specimen   being   observed. A   system   of   knobs   on   the   side   of   the   stage   allows   you   to   move   the   specimen   under   the   objective   on   the  X  and  Y  axes. Try  and  move  the  stage. COARSE   FOCUS   KNOB:   Permits   rapid   change   in   distance   between   the   specimen   and   the   objective   thereby   allowing   for   rough   focussing   –   Do   not   use  when  focusing  with  the  40x  objectiveFINE   FOCUS   KNOB:   Permits   small   changes   in   distance   between   the   specimen   and   the   obje ctive   and   thereby   allows   for   final   focussing   of   the   image. 10 Lab1  ? Microscopy OCULAR   OR   EYEPIECE:   A   magnifying   element   in   the   microscope,   usually   10X. It   is   through   the   ocular,   or   eyepiece   that   one   looks   at   the   specimen. All   our   microscopes   are   parfocal,   so   that   when   an   object   is   in   focus   with   one   objective,   the   focus   will   not   be   completely   lost   when   changing   to   the   next  objective. OBJECTIVES:  The  magnifying  element  which  is  closest  to  the  specimen.See   figure  1  to  find  out  about  the  engravings  on  the  side  of  each  objective. CONDENSER:   System   of   lenses   that   concentrates   the   light   furnished   by   the   illuminator. It  does  not  magnif y  the  object. CONDENSER   HEIGHT   ADJUSTMENT   KNOB:   Allows   one   to   focus   the   concentrated  light  onto  the  specimen. APERTURE  IRIS  DIAPHRAGM:  Used  to  reduce  glare  from  unwanted  light  by   adjusting  the  angle  of  the  cone  of  light  that  comes  from  the  condenser; Production  of  Image  by  a  Compound  Microscope The   most   important   part   of   a   microscope   is   the   objective.All   the   other   parts  of  the  instrument  are  designed  to  help  the  objective  produce  the  best   possible  image. The  best  image  is  not  the  largest;  it  is  the  clearest. There  is   no   value   to   a   high   magnification. If   the   resolution   is   poor   you   will   have   no   better  understanding  of  the  specimen. light  beam ocularà ‚  lens Magnification Numerical  aperture  (NA) Determines   the   resolving power  of  the  objective* Optical   tube   length   /   max. coverslip  thickness  in  mm prism objective  lens specimen condenser  lens Figure  1:  Objectives  engravings light  sourceFigure  2:  Image  production  in  a  compound  microscope. 11 Lab1  ? Microscopy *Resolving  power  is  the  ability  to  see  two  objects  that  are  very  close  as  two   separate  objects. The  human  eye  will  resolving  power  is  about  100 µm. Using  the  compound  microscope Always   handle   the   microscope   GENTLY! It   is   an   expensive,   delicate   and   heavy   instrument. Carry   it   with   two   hands,   one   hand   on   the   arm,   and   the   other   hand   under   the   base. If   the   ocular   or   objective   is   dir ty,   wipe   it   clean   using   ONLY   Kimwipes   or   special   lens   tissue   and   cleaning   fluid   supplied.If   you   use   anything   else   you   may   scratch   the   lens. Wipe   up   any   cleaning   fluid   immediately;   otherwise   it   will   dissolve   the   glue   which   holds   the   lens   in   place. REMEMBER,  your  demonstrator  is  here  to  help,  so†¦ ASK! 1. Make   sure   that   the   power   cord   is   plugged   into   the   back   of   your   microscope  and  into  a  power  outlet. 2. Using  the  letter  Ã¢â‚¬Å"e†Ã‚  microscope  slide  provided,  follow  steps  2  through  13   in   the   Setup   and   Bright   field   alignment   procedure   of   Biolabo. Remember,   observation  can  be  done  on  screen  or  through  the  oculars. Orientation  an d  working  distance . Starting   your   examination   with   the   4X   objective,   position   the   letter   â€Å"e†Ã‚   slide  on  the  stage. 2. Draw  what  you  see  in  the  microscope:_________________ 3. What  would  a  slide  with  the  letter  Ã¢â‚¬Å"t†Ã‚  look  like  under  the  microscope? _________________ 4. Using  the  knobs  located  on  the  side  of  the  stage  and  looking  through  the   microscope,   move   the   slide   slowly   to   the   right,   then   to   the   left. Record   your  observations. ___________________________________   5. Now,   move   the   slide   slowly   away   from   you,   then   towards   you   while   observing  through  the  microscope.Record  your  observations   ____________________________________ 6. Focus  on  the  slide  at  10X. Check  the  dist ance  between  the  objective  lens   and  your  slide  (=  the  working  distance,  see  also  the  reference  at  the  end  of   this   chapter). Now   switch   to   the   40X   objective   and   check   the   working   distance. What   happens   to   the   working   distance   as   your   magnification   increases? 12 Lab1  ? Microscopy Depth  of  field  (depth  of  focus) Lenses   have   a   depth   of   focus. It   is   the   number   of   planes   in   which   an   object   appears   to   be   in   focus.Extend   your   fist   at   arm’s   length   in   front   of   you   and   hold   your   thumb   up. Concentrate   on   your   thumb   and   notice   that   the   objects  past  your  thumb  on  the  other  side  of  the  room  are  not  clearly  seen. Similarly   withà ‚   a   microscope,   when   it   is   focussed   on   one   surface,   the   surfaces  lower  or  higher  will  be  out  of  focus. 1. Position   a   prepared   slide   with   coloured   threads   upon   the   stage. At   low   power,  4X,  focus  on  the  area  where  the  threads  cross. 2. Using  the  fine  focus  adjustment,  focus  up  and  down  slowly. 3. Repeat   using   different   objectives.What   can   you   say   about   the   depth   of   field   at   different   magnifications? Has   it   increased   or   decreased? (i. e. ,   can   you  see  more  threads  in  one  focal  plane  at  4X  or  40X? ) ____________________________________________________________ Magnification The  magnification  given  by  objectives  and  oculars  is  engraved  on  them. The   total   magnification   for   any   combination   of   objective   and   ocular   is   the   product  of  the  magnification  of  each  lens. Objective  magnification Ocular  magnification Total  Magnification Light  intensity Working  distance 4x 10x 40x High 22mm 10x 10x 100xMedium 10. 5mm 40x 10x 400x Low 0. 56mm Table1   . Comparison   magnification,   working   distance   and   brightness   at   three   different   objective  magnifications. You  also  can  calculate  the  magnification  of  your  picture  using  the  following   formula: Magnification  factor=  measured  size  of  object  =  (  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  X) Actual  size  of  object 13 Lab1  ? Microscopy Specimen   size   and   Magnification   of the  picture Before   you   start   this   exercise,   make   sure   you   have   carefully   read   the   website   section   rele vant   to   the   software   you   will   use   to   take   digital   pictures  (ImageJ/Qcapture).The   goal   of   this   section   is   to   teach   you   different   techniques   that   will   allow   you   to   determine   the   size   of   objects   you’re   observing   under   the   microscope. The   general   principle   is   fairly   simple:   2   objects   have   the   same   relative   size   (expressed   as   a   ratio)   in   the   real   world   and   under   the   microscope. actual  size  of  object  A  Ã‚  Ã‚  =  on? screen  size  of  object  A  Ã‚  ? A1  =  A2     actual  size  of  object  B  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  on? screen  size  of  object  B  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  B1  Ã‚  Ã‚  Ã‚  B2 The  following  exercises  are  applications   of  this  formula. Place  a  sli de  under   the   microscope.Choose   the   right   objective   and   adjust   the   focus   and   light   level. Then,  choose  a  structure  you  want  to  measure  and  take  a  picture. A? First   method:   Measuring   an   object   using   the   field   of   view  (FOV): The   simplest   way   to   determine   the   size   of   an   object   is   to   use   the   known   size  of  the  whole  field  of  view  (FOV,  the  whole  picture  from  left  to  right). 1? On   the   computer   screen   (using   a   ruler   and   without   writing   anything   of   the  screen),  measure  the  object  of  which  you  want  to  determine  the  size  (=   A2) 2? Then,  measure  the  width  of  the  whole  picture  on  the  screen  (=B2). ? Refer   to   table   2   on   page   20   to   know   the   actual   size   of   the   field   of   view   for  the  objective  you’re  using  (=B1) 4? Use  the  following  formula: Actual  size  of  the  object  (A1)  =  Actual  size  of  the  FOV  (B1)  Ã‚  Ã‚  Ã‚  Ã‚  x  Ã‚  Ã‚  on? screen  size  of  the  object  (A2) on? screen  size  of  the  FOV  (B2) Example:   On   a   snapshot   using   the   4x   objective,   an   insect   has   an   on? screen   length  of  10cm. The  whole  picture  is  20cm  wide. What  is  the  actual  size  of  the  insect? ______________________________ 14 Lab1  ? Microscopy B? Second   method:   Measuring   an   object   using   a   scale   bar  file:From   Image   J   (using   the   file   /   open   command),   open   the   file   that   contains   the   relevant   scale   bar   in   the    (T:/BIO/BIO1140):   new10X. jpg   for   the   10x   objective,  and  new40X. jpg  (for  the  4x  and  40x  objectives). Then,   using   a   ruler   measure   the   following   distances   directly   on   the   computer  screen: 1? The   on? screen   length   (or   width)   of   the   object   whose   size   you   wish   to   determine  (=A2) 2? The  width  of  the  scale  bar  on  the  screen  (=B2)   You  now  can  calculate  the  actual  size  of  the  object  using  the  formula: actual  size  of  object  =  on? creen  length  of  object  Ã‚  x  Ã‚  actual  size  of  scale  bar*        Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  on? screen  length  of  scale  bar ?  Ã‚  Ã‚  Ã‚  A1  =  A2  x  B1 B2 *The   actual   size   of   the   scale   bar   is   indicated   on   the   scale   bar   file   (ex:   on   the   new10x. jpg   file,  the  bar  represents  0. 2mm  at  10x  or  0. 02mm  at  100x)  =  B1 Example:  I  took  a  picture  of  a  small  insect  larva,  using  the  4x  objective. The   larva   length   is   60mm   on   the   screen. The   scale   bar   on   the   new40x. jpg   is   30mm  and  represents  0. 2mm. What  is  the  actual  size  of  the  larva? _________________________Do  not  put  the  compound  microscope  back  in  the  cupboard  you  will  need  it   later  this  afternoon. Points  to  remember  concerning  microscopes 1. Always   work   with   a   clean   microscope. Use   only   the   lens   paper   provided. Don't  forget  to  clean  the  slide  too! 2. Always   locate   the   specimen   under   low   power   and   work   your   way   up   to   the  high  power  objective. 3. Never   use   the   coarse   focusing   knob   when   the   high   power   lens   is   in   position. Use  only  the  fine  focus  knob. 4. Never  use  the  100x  in  1st  year  labs  (we  didn’t  teach  you  how)   5.Always   readjust   illumination   whenever   you   change   the   objective. Too   much  light  will  give  you  a  blurry  image  that  you  cannot  focus  on. 15 Lab1  ? Microscopy The  stereoscopic  microscope (dissecting  microscope) The   stereoscopic   microscope,   also   called   stereoscope   or   dissecting   microscope,   is   used   to   view   objects   that   are   too   large   or   too   thick   to   observe  under  the  comp ound  microscope. Stereo   microscopes   are   always   equipped   with   two   oculars   producing   a   stereoscopic   or   three? dimensional   image. Unlike   the   compound   microscope,  the  image  is  not  inverted.Our   stereo   microscopes   provide   magnification   in   the   range   of   6. 7X   ? 45X   using   a   zoom? type   lens   system. By   rotating   a   dial   located   on   the   right   side   of   the   stereo   microscope   head,   the   viewer   obtains   a   continuous   change   of   magnification. Our   stereo   microscopes   can   be   used   with   reflected   or   transmitted   light. Reflected   light   is   directed   unto   opaque   specimens   from   above   and   is   reflected   to   the   viewer. Transmitted   light   is   used   with   translucent   specimens   and   passes   through   the   specimen   from   beneath   the   stage   and   into  the  viewer's  eyes.Use  of  the  stereoscopic  microscope 1. On   the   Biolabo   home   page   left   click   on   Stereoscope   (Dissecting   microscope)  and  then  on  Stereoscope  setup. 2. Click  on  Step  1  and  read  it  carefully. Obtain  a  stereo  microscope  from  the   same  cupboard  as  your  compound  microscope  if  you  haven’t  yet. 3. Click  on  and  read  steps  2  through  7. 4. Place  a  coin  on  the  stage. 5. Using   the   focussing   knob   on   either   side   of   the   arm,   lower   or   raise   the   objective   until   the   coin   is   in   focus. Examine   it   in   both   reflected   and   transmitted  light.Which   is   best   for   an   opaque   specimen? Try   t he   various   magnifications   by   turning   the   zoom   knob. The   reflected   light source   is   similar   to   a   spotlight   and  its  orientation  can  be  adjusted  manually. Try  rotating  the  light  upwards   and  downwards. 6. Examine  other  materials  such  as  brine  shrimp  larvae  (Artemia)  in  a  watch   glass   using   both   reflected   and   transmitted   light. Add   1? 2   drops   of   â€Å"proto? slow†Ã‚   solution   to   slow   down   the   larvae. Estimate   the   actual   size   of   one   larva:  __________ 16 Lab1  ? Microscopy Prokaryotic  and  Eukaryotic  cellsIt   has   long   been   recognized   that   living   organisms   are   composed   of   basic   structural   and   functional   units   called   cells. Cells   can   be   divided   into   two   general   type s:   prokaryotic   and   eukaryotic,   based   on   the   presence   of   a   nucleus  and  other  membrane  bound  organelles  in  the  latter. Prokaryotic   cells   belong   to   2   big   groups:   archaea   and   eubacteria. They   are   usually   smaller   than   eukaryotic   cells   (typically   1? 5 µm). These   unicellular   organisms  may  be  small,  but  they  are  the  most  abundant  organisms  on  the   planet,   representing   about   half   the   biomass   (Biology,   Brooker   et   al. 010,   McGraw? Hill&Ryerson). They   are   devoid   of   membrane   bound   organelle   such   as   the   nucleus,   mitochondria   or   chloroplasts. Their   genetic   material   is   usually   composed   of   one   circular   chromosome   plus   other   extra   chromosomal  elements  called  plasmids. Euka ryotic   cells   are   usually   much   larger. They   possess   a   membrane   bound   nucleus,   their   organelles   are   more   complex   and   numerous,   and   their   genome   is   larger   than   prokaryotes. Eukaryotic   organisms   can   be   uni? or   multicellular. You   will   have   a   chance   to   observe   many   eukaryotic   cells   during  this  semester:  Amoeba,  Lilly,  Whitefish†¦.In   today's   exercise   you   will   take   a   first   look   at   the   similarities   and   differences   between   prokaryotic   and   eukaryotic   cells   as   well   as   the   diversity  within  these  groups. You   should   familiarize   yourselves   with   a   whole   array   of   cellular   structures   and   organelles   you   will   probably   encounter   during   the   course   of   this   exercise. Before   your   scheduled   lab   session,   write   down   the   definition   and   function  for  each  of  the  following  terms:  plasma  (cell)  membrane,  cell  wall,   protoplast,  cytoplasm,  vacuoles,  nucleus,  nucleolus  and  chloroplasts.Eukaryotic  Cells:  Elodea  (plant) 1? Get   a   young   green   Elodea   leaf   from   the   jar. Mount   it   in   a   drop   of   water   on   a   clean   microscope   slide   with   the   convex   side   of   the   leaf   uppermost. Cover  the  preparation  with  a  coverslip. 2? Observe   the   preparation   at   4X,   then   at   10X. If   you   see   brownish   oval   structures   on   the   leaf   surface,   ignore   then. These   are   probably   epiphytic   diatoms. Concentrate  your  attention  on  the  cells  near  the  cent ral  rib  at  the   base  of  the  leaf  and  on  the  marginal  cells  at  the  edge  of  the  leaf. Can  you  distinguish  several  layers  making  up  the  leaf? ____ ? What   is   the   average   length   ______   and   width   ______   of   the   cells   in   micrometres? 17 Lab1  ? Microscopy 3? Focussing  at  40X  locate  the  cell  wall,  the  vacuole,  the  cytoplasm  and  the   numerous  green  chloroplasts. ? What   important   biological   process   takes   place   in   the   chloroplasts? _____________________________________ ? What  pigment  is  responsible  for  their  green  colouration? ________________________________________________ ? What  is  the  shape  of  chloroplasts? ____________________________________________ ? Are  the  chloroplasts  moving? What  sort  of  movement? ____________________________ _____________________ ? The  phenomenon  you  are  observing  is  called  cytoplasmic  streaming   or   cyclosis. What   do   you   think   the   function   of   such   a   process   could   be? ___________________________________________________ 4? You   have   probably   realised   that   the   plasma   membrane   cannot   be   seen   in   plant   cells. It   is   too   thin   to   be   resolved   with   the   compound   microscope.In   order   to   see   the   true   limiting   boundary   of   the   cytoplasm   it   is   necessary   to   treat   the   cells   in   such   a   manner   that   the   plasma   membrane   becomes   withdrawn   away   from   the   rigid   cell   wall. This   can   be   done   by   placing   the   cell   in   a   strong   salt   solution. This   will   cause   water   toà ‚   diffuse   out   of   the   cell   by   osmosis,   thereby   decreasing   the   cell   volume. The   unaffected   cell   wall remains  in  its  original  state. What  can  then  be  seen  is  a  space  between  the   cell  wall  and  the  limiting  boundary  of  the  protoplast  (the  cell  minus  the  cell   wall)  which  thereby  becomes  visible. Remove   your   Elodea   slide   from   the   microscope   stage. Delicately   remove   the   coverslip,   add   one   drop   of   5%   NaCl   solution   then   put   back  the  coverslip  on  your  preparation ? Refocus   at   40x   (don't   forget:   you   must   first   focus   at   4X,   then   10X   and  finally  at  40x). ? Are   the   cells   plasmolyzed? (If   not   wait   a   while   longer). How   do   they   look  like  now? __ ____________________ ? Has  the  cell  wall  been  affected? _________________   ? What   becomes   of   the   large   central   vacuole   during   plasmolysis? ______  _______________________________________________ Take  a  picture  of  a  plasmolyzed  Elodea  cell. How  does  it  compare  to   the  previous  picture? 18 Lab1  ? Microscopy Prokaryotic  Cells:  Lyngbya  (eubacteria:  cyanobacteria)   1. Take   a   close   look   at   the   sample   in   the   jar. Which   colour   would   best   describe  its  appearance? ___________________ 2. Prepare  a  wet  mount  of  fresh  Lyngbya  by  the  following  procedure:   ? With   forceps   or   an   eye   dropper,   put   a   very   small   amount   of   green   matter  on  a  clean  slide ? Add  a  drop  of  water  from  the  jar. ? Carefully   place   a   co verslip   over   it. Make   sure   it   lies   flat   on   the   preparation.Don't   worry   if   there   are   just   a   few   air   bubbles. With   practice,   your   skills   will   improve. However,   if   too   many   air   bubbles   are   present,   your   preparation   risks   to   dry   out   very   quickly   during   viewing,  compromising  your  observations. 3. Starting  with  the  4X  objective,  focus  on  your  preparation. ? Can  you  see  numerous  green  filaments? _______ ? Are  the  filaments  moving? __________ 4. Switch  to  the  10X  then  the  40X  objective  and  focus  using  the  fine  focus   knob  only: ? Do  you  see  the  individual  cells  making  up  each  filament? ________ ? Estimate  the  width  of  one  filament  in  micrometres:_______ What’s  the  filamentà ‚  width  in  millimetres  (mm)? ________ ? REMEMBER:   You   are   working   with   living   cells. Work   quickly   and   keep   your   specimen   wet   at   all   times. Dead,   dry   or   damaged   biological  preparations  are  useless. Returning  the  microscopes  after  use After   completing   all   observations,   turn   and   click   the   low   power   objective   (4X)  on  the  compound  microscope  into  position. Remove  the  slide  from  the  stage  and  return  it  to  its  correct  box. Wipe  the  stages  with  a  clean  paper  towel. Carefully  disconnect  the  camera  from  the  firewire  cable.Make   sure   you   turned   off   the   light   on   each   microscope,   then   unplug   the   power  cord  and  make  a  loose  coil  of  it  around  the  eyepieces. Return  the  microscope  in  the  cupboard. 19 Lab1  ? Microscopy TAs   will   check   that   you   properly   returned   the   microscopes   in   the cupboard   with  the  cord  properly  attached  and  no  slide  present  on  the   stage. You   will   lose  marks  for  this  lab  (and  other  labs)  if  you  don’t  do  so. Evaluation A   short   quiz   on   microscope   components,   specimen   observations   and   measurement  of  objects  will  take  place  at  the  beginning  of  Lab2.Be  on  time,  the  quiz  will  start  at  2:30. References: 1? Metric  system  (see  also  appendix  IV  at  the  end  of  lab  manual):   1  centimetre  cm  =  10? 2  metres  (m) 1  millimetre  mm  =  10? 3  metres 1  micrometre  ? m  =  10? 6  metres 1  nanometre  nm  =  10? 9  metres 2? Size  of   camera  field  of  views  (fov): Table  2:  Fields  of  View:  Olympus  CX41  Compound  Microscope   Objective 4X 10X 40X 100X Camera  field  of  view (width  in  mm) 1. 75 0. 70 0. 175 0. 070 Table  3:  Fields  of  View  Ã¢â‚¬â€œÃ‚  Olympus  SZ61TR  Dissecting  Microscope   Zoom  Setting 0. 67X 0. 8X 1X